Composite

Part:BBa_K4619010:Design

Designed by: KaMing Li   Group: iGEM23_UCAS-China   (2023-10-09)


PobR-Threshold Guard Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal AgeI site found at 154
    Illegal AgeI site found at 675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 595
    Illegal SapI.rc site found at 1573


Design Notes

The assembly process is as follows: The original pobA/R operon consists of six main components: pobA, pobA RBS, pobA/R dual-directional promoter, pobR operator, pobR RBS, and pobR. The pobA gene encodes an unnecessary enzyme that breaks down 4-HBA. Therefore, we remove the pobA and pobA RBS components but retain the entire promoter, pobR RBS, and pobR.

Source

[1] Calles B , Goni-Moreno A , Lorenzo V D .Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module[J]. 2019.DOI:10.1101/783506.

References